%0 Dataset
%T Analysis data of soil bacterial community structure of typical sand fixing shrub land in Maowusu Sandy Land (2016)
%J National Cryosphere Desert Data Center
%I National Cryosphere Desert Data Center(www.ncdc.ac.cn)
%U http://www.ncdc.ac.cn/portal/metadata/3b86d62d-2a4f-40a5-89e2-48a78f9ba501
%W NCDC
%R 10.12072/ncdc.ZDYF.db1679.2022
%A None
%A Sun Yanfei
%K Mu Us sandy land;sand fixing shrub;soil bacteria
%X According to the principles and methods of soil microbial research by Lin Xiangui and others, combined with the requirements of the project, a unified sampling specification and method are formulated for sampling. In Mu Us sandy land, three representative sand fixing shrub forests planted in the same year, Artemisia annua, Salix psammophila and Caragana korshinskii, are selected. Three 20 m shrubs are randomly set in each shrub forest × For the sample plot of 20m, the spacing between various plots is 20m. At the same time, the bare sand is selected as the control at the place less than 20m away from the shrub forest land.Soil samples were taken in August when plants grew most vigorously and microbial activities were most active. In each sample plot, 5 shrubs (clumps) are randomly selected, and the sterilized soil drill with a diameter of 2.5cm is used to take the soil within the depth range of 0-10cm under and between shrubs respectively. The surface plant litter is removed before collecting samples. Mix the soil samples collected from each sample plot into a mixed sample (about 1000g) according to the soil samples under and between shrubs, put them into sterile plastic bags, store them in the incubator, and quickly transport them back to the experimental station. The mixed soil samples are screened for 2mm to remove roots and litter. Each screened soil sample was immediately stored at - 20 ℃ for high-throughput sequencing.